(This question has already been solved BUT this question is about why the answer is A instead of E)
The question asks for complementary bases of a DNA plamid cut with a single restriction enzyme.
The sticky end on the left is A-A-C-G-A, which normally gives a complementary sequence of T-T-G-C-T which is the correct answer in the text. BUT if we’re talking about a plasmid that has to be closed, why isn’t the correct sequence in the reverse order (TCGTT), because when you close the plasmid the outermost base pair goes to the innermost base pair on the opposite end (I’ve attached a picture for clarity, I added the numbers in blue to explain what I mean, why doesn’t 1 go with 1, 2 with 2, 3 with 3 etc.)
thank you SO MUCH