IMAT 2020 Q32 [Recombinant DNA]

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The diagram shows how recombinant DNA can be used to make cotton plants produce an insect toxin. The insect toxin kills any insect that can damage the cotton crop.

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What are the TWO functions of the Agrobacterium tumefaciens bacteria in this process?

  1. They stimulate cell division in cotton plant seeds.
  2. They supply the plasmid vector.
  3. They are the source of the gene for the production of the insect toxin.
  4. They are the source of the gene for antibiotic resistance.

A. 1 and 3 only
B. 1 and 4 only
C. 2 and 3 only
D. 1 and 2 only
E. 2 and 4 only

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What is the correct answer?

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Here the answer is D as the Agrobacterium tumefaciens bacteria contains the plasmid vector which provides the desired gene for the toxin . The first option might be looking irrelevant but it has a deep meaning. The Agrobacterium tumefaciens promotes the cell division of cells which has the GOI of the toxin . So it indirectly represents that it supports cell division in the plants .