Imat past paper 2019 Q32

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Hi!
Can someone explain how to solve this? Answer is E.

Hi!
The question says the red pigment does not cross the cell membrane, thus the water should not turn red when the beetroot is placed solely in water that is just at 20 degrees.
We can thus rule out row 1, 4.
Ethanol breaks down phospholipids and the cell membrane (thus the water will turn red).
HCl will also break down the cell membrane (thus the water will turn red) and HCl lowers pH which can denature proteins.
Row 3 is thus correct.
Row 2 is not correct because ethanol doesn’t denature proteins.
Hope it will help:)

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