Restriction enzymes

Practice Question Solving
1

Restriction enzymes derived from bacteria are used in recombinant DNA techniques. The enzyme BamHI cuts DNA in the sequence
G↓
G─A─T─C─C
C─C─T─A─G ↑
G
When used to cut the sequence
5’…ATGGATCCGGACTAA…3’
3’…TACCTAGGCCGGATT…5’
How many DNA fragments are formed?
A) 2 with flat ends
B) 3 with cohesive ends
C) No fragments are formed
D) 2 with cohesive ends
E) 3 with flat ends

The answer is D. Could anyone help?

2

I think, It must be 3 or the multiples of 3 BUT I’m not sure :slight_smile:
RCWkiM0J8yg3VHImeLkQrw

3

5’…ATG GATCCGGACTAA…3’
3’…TACCTAG GCCGGATT…5’

Oh, I wasn’t sure of the terminology, but based on your image, it looks like it should be split in two like this and has two sticky ends?